AllGlo™ technology includes our AllGlo probes and primers, and has been developed to meet the challenges of cost, performance and reliability facing fluorogenic real-time PCR reagents in today's market place. Because of the unique designs, AllGlo probes and primers rival their competitions in many important areas. In its simplest form, an AllGlo probe contains two identical reporter dyes capable of quenching their own fluorescence prior to hybridization to its target sequence or before enzymatic cleavage of the probe. Because AllGlo probes do not employ a quencher and have two signal-generating reporter dyes per oligo, they are much simpler to manufacture and, in most of the cases, offer much higher sensitivity than traditional TagMan probes, including TaqMan probes with a MGB group, which are far more complex and thus expensive to synthesize.

Central to AllGlo™ technology is our proprietary AllGlo fluorescent dyes, which are available in many colors encompassing wavelengths from UV to infrared, making multiplex detection easily attainable.

To learn more about AllGlo™ technology, you can download the following information sheets:

AllGlo™ Highhights	125 Kb
AllGlo™ Information Brochure	265 Kb
AllGlo™ Slides - CHI's PCR & Beyond, McLean, VA July 21-23, 2004	804 Kb

Q. What is AllGlo technology?

A. AllGlo technology is a simple and sensitive fluorescence-based real time quantitative PCR system developed at AlleLogic BioSciences. The system doesn't require a quencher. It employs two identical fluorescent dyes that are attached to a single fluorogenic oligonucleotide. The unique designs of the dyes and linkers, as well as the selection of attachment sites, promote fluorescence quenching prior to the hybridization and probe cleavage. Upon hybridization with the target sequence, the labeled oligo becomes stretched and cleaved. This leads to the separation of the two reporter dyes and de-quench occurs. As this process releases two fluorescing molecules instead of one, the signal change is much greater, making AllGlo-based quantitative PCR detections highly sensitive and robust.

Q. Why AllGlo probes generate higher signals?

A. Each AllGlo probe contains two identicle fluorogenic reporter dye, therefore it can generate twice as much signal as all other probe systems.

Q. Can AllGlo technology be used in fluorogenic probes or fluorogenic primers?

A. It can be used in both forms.

Q. Can my TaqMan, TaqMan MGB, MGB Eclipse Probe assays be converted to AllGlo probe assays?

A. Yes, just replace the fluorophore and quencher with a single AllGlo dye. What TaqMan, TaqMan MGB, MGB Eclipse Probe assays can do, AllGlo probes can do too with improved performance.

Q. What do I need to change in order to use AllGlo probes?

A. Not much. Basically you can keep the same conditions, which include realtime theromocycler, cycling condtion, enzyme, buffer system, primers and the probe sequence.

Q. Is AllGlo technology compatible with other Tm-increasing technologies?

A. Yes, regular AllGlo probes use regular nucleotide. It can be used in conjunction with minor groove binders, superbases, locked nucleic acid, and peptide nucleic acid.

Q. Do I need a special probe design program?

A. No. Most public and commercially available design software can be used for choosing AllGlo probes and primers. Try to avoid base G at the 3' end of oligo.

Q. How many coloers can be used in AllGlo Probes?

A. A variety of fluorophores are currently available, more dyes to come:

Q. Are AllGlo probes difficult to synthesis?

A. No. In contrast, due to its simple design, AllGlo probes are much easier to make and purify.